Sunday 29 May 2016

Palindromic DNA implies | Crack PGIMER NOV 2016


Palindromic DNA implies

A. Short stretches of DNA
B. Recognized by specific restriction endonuclease
C. Codes for bacterial resistance
D. Complementary strands
E. part for microbial genetic drifts

The Answer is(A) Short stretches of DNA; (B) Recognised by specific restriction endonuclease (D)
Complementary strands
• A palindrome is a region of double stranded DNA in which each of twa strands has same sequences when read in the same direction (i.e. in the 5’- 3’ direction)
• They have complementary strands
• Restriction endonuclease also called Restriction enzymes cleave double stranded DNA into smaller, more manageable fragments, opened for DNA analysis. These enzymes recognize short Stretches of DNA (usually 4 to 6 base pairs) that contain specific nucleotide sequences. Thus, sequence which differ for each restriction endonuclease, are palindromes.
• Drug resistance is due to
- Chromosome mediated resistance
- Plasmid mediated resistance
-Transposon mediated resistance