Friday 20 May 2016

Processes inhibited by the presence of extracellular nucleases | Crack PGIMER 22 MAY 2016


Which of the following processes is most likely to be inhibited by the presence of extracellular nucleases? 

A. Conjugation 
B. Generalized transduction 
C. Specialized transduction 
D. Transformation 
E. Transposition


The answer is. D. Transformation

In transformation, the DNA is extracellular before it is picked up by the competent cells; during this period, the DNA is subject to the extracellular endonucleases. Because the DNA in generalized and specialized transductions is protected extracellularly by the virus capsid, it is not subject to extracellular endonucleases. In conjugation, the DNA is never outside of a cell. Transposition is a mechanism of inserting a transposon into another molecule of DNA and has no extracellular transport mechanism associated with it.