In general, enzyme functions as
A. ed activation energy
B. ed chemical reaction rate
C. Change the equilibrium constant of reaction
D. ted the substrate concentration at active site
E. Change the total energy of the reaction
A. ed activation energy
B. ed chemical reaction rate
C. Change the equilibrium constant of reaction
D. ted the substrate concentration at active site
E. Change the total energy of the reaction
Ans. A. ↓ed -Activation energy; (B) ↑edChemical reaction rate;
• The enzyme acts by:
* Lowering
ofActivation Energy
* Forming enzyme substrate complex (ES) which divides to enzyme and
product
(Michaelis-
Menten Theory)
E+S—>ES—> E+S
* Enzyme — Substrate fit each other similar to lock and key (Fischer’s template Theory)
* The Substrate induces conformational changes in the enzyme, such that
precise orientation of catalytic group is effected. (koshland’s induced fit theory)
* Proximity
effect of enzyme :Enzyme Substrate complex formation can improve collision
probabilities manifold , causing the rapid
reaction rate.
* Product substrate orientation.
* Strain theory.
* Rack theory.
• At active site specific substrate is bound and this binding depends on alignment
of specific group or atoms at active sites.
